EMPIRI INC.
Single-Cell RNA Sequencing
Services we provide
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End-to-end or à la carte service, including fresh tissue handling to final report
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Custom study design consultation
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Sample pick up service from the Texas Medical Center area for rapid sample processing
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Cloud-based bioinformatics Pipeline for basic and custom computational analysis
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Specialize in clinical trial samples, including biospy core analysis
Sample Collection
Tissue Dissociation into single cells
Single cell capture and library generation
Sequencing
Bioinformatic Analysis
Data storage
UMAP visualization of clusters from scRNA Sequencing analysis
Single Cell Sequencing Service
The Workflow
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We provide a comprehensive end-to-end single-cell RNA sequencing service, encompassing tissue dissociation through to bioinformatics analysis. Our droplet-based single-cell RNA sequencing service, which includes immune profiling, enables the deciphering of the intricate complexities within each cancer ecosystem. By elucidating cellular heterogeneity—specifically in terms of cell type composition and activation states before and after treatment—this service offers profound insights into cellular and molecular dynamics.
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Services Offered:
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Single Cell Gene Expression 3' and 5'
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Single Cell Immune Profiling (TCR/BCR sequencing)
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Single cell ATAC-seq
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Spatial-sequencing (Visium)
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Total RNA seq
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The Latest in scRNA Sequencing
Early insights into clinical trials through single-cell RNA sequencing from biopsy cores​
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Single cell RNA-sequencing (scRNA-seq) yields valuable insights into the molecular
heterogeneity of multiple cell types in normal and cancer tissues
Immunity, Jun 2021
Haley L Dugan et al.
PubMed: 33870241
Profiling B cell immunodominance after SARS-CoV-2 infection reveals antibody evolution to non-neutralizing viral targets​
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Single-cell sequencing was used to describe SARS-CoV-2 B cells, and revealed "antibody adaptation to non-neutralizing intracellular antigens during infection".
Cell Stem Cell, Jun 2021
Ling Huang et al.
PubMed: 33915081
Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids
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Organoids mimicking the exocrine pancreas were developed to model "lineage tropism and plasticity for oncogene action in the pancreas". Single-cell RNA sequencing was used to asses molecular differences between organoid lineages.
Nature, Jun 2021
David Liu et al.
PubMed: 33941922
Evolution of delayed resistance to immunotherapy in a melanoma responder
Single-Cell RNA sequencing data along with immunofluorescence was used to understand immune resistance in melanoma patients, revealing "differences in immune composition among different lineages".
Nature, Jun 2021
Daniela J Di Bella et al.
PubMed: 34163074
Molecular logic of cellular diversification in the mouse cerebral cortex
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An atlas of a developing mouse-neocortex was created using single-cell RNA sequencing data to help show "lineage-specific developmental abnormalities" commonly linked to mutations.